. 2007 Oct;13(10):1574–1578. doi: 10.3201/eid1310.070158

Table 2. Individual amino acid substitutions of Glubinnoe/2004 and 205/Sofjin-HO viruses and unique substitutions in comparison with 37 flaviviruses, determined by polyprotein alignment*†.

Gene	Amino acid substitutions Glubinnoe/2004 → 205  or Sofjin-HO		Unique substitutions	Changed putative cleavage sites	Summary of substitutions 205/Sofjin-HO
Gene	205	Sofjin-HO	Unique substitutions	Changed putative cleavage sites	Summary of substitutions 205/Sofjin-HO
Viral C	No	M₄₃ → L; A₅₄ → V; N₆₄ → K	No	No	0/3
CTHD	A₉₉‡ → V; I₁₀₈ → V;  M₁₁₁ → I; M₁₁₃‡ → V; F₁₁₅‡ → L	A₉₉‡ → V; I₁₀₈ → V;  M₁₁₁ → L; M₁₁₃‡ → V;  F₁₁₅‡ → L	No	Viral C/CTHD (RGKRR/SAA†DW) anchored C/prM (GM‡TF‡A/ATVRK)	5/5
prM	No	No	No	No	0/0
M	R₂₁₀‡ → P	R₂₁₀‡→ P; I₂₄₆ → M; I₂₆₆→ V	R₂₁₀*	prM/M (SRTRR/SVLIR‡)	1/3
E	K₅₀₈ → R; I₅₉₇ → T; T₆₄₆ → N; V₇₄₃ → A	A₄₃₃ → V; T₆₄₆ → N; M₇₄₀ → V; G₇₅₉ → S	No	No	4/4
NS1	G₇₈₀‡ → C; K₈₈₃ → R; S₉₅₁ → P; I₁₀₅₃ → T	I₁₀₅₃ → T; V₁₁₂₂ → I	No	E/NS1 (LGVGA/DVGG‡A)	4/2
NS2A	R₁₁₈₀ → K; R₁₂₂₇ → S; G₁₂₅₀ → S; G₁₂₇₇ → E; C₁₃₁₁ → Y	R₁₁₈₀ → K; R₁₂₂₇ → S; G₁₂₅₀ → S; G₁₂₇₇ → E; I₁₂₉₆ → T; A₁₂₉₇ → V	No	No	5/6
NS2B	V₁₄₂₃ → M	V₁₄₂₃ → M	No	No	1/1
NS3	E₁₅₆₃ → D; G₁₆₅₀ → E; I₁₆₇₃ → S; I₁₇₀₇ → T; T₁₈₂₈ → S; A₁₈₆₁ → V; P₁₉₄₈ → Q; V₁₉₇₅ → G; D₁₉₈₈ → N; A₂₀₆₂ → T	D₁₄₉₁‡ → G; S₁₅₅₁ → Y; E₁₅₆₃ → D; G₁₆₅₀ → E; I₁₆₇₃ → T; N₁₇₃₁ → S; T₁₈₂₈ → S; T₁₈₆₉ → A; P₁₉₄₈ → Q; V₁₉₇₅ → G; D₁₉₈₈ → N; A₂₀₆₂ → T	E₁₅₆₃; G₁₆₅₀; P₁₉₄₈; V₁₉₇₅; D₁₉₈₈	NS2B/NS3 (RTARR/SD‡LVF)	10/12
NS4A	D₂₁₄₃ → E ;V₂₁₆₅ → A	D₂₁₄₃ → E; A₂₁₇₃ → G	D₂₁₄₃	No	2/2
NS4B	M₂₂₈₃ → L; I₂₃₁₄ → M; A₂₃₃₁ → V; V₂₃₄₉ → I; A₂₄₇₂ → V	M₂₂₈₃ → V; F₂₃₄₇ → L; S₂₄₅₇ → A	M₂₂₈₃	No	5/3
NS5	K₂₆₂₅ → R; A₂₇₅₇ → G; G₂₇₅₈ → D; E₃₀₁₃ → G; S₃₀₁₄ → F; G₃₀₃₃ → E; K₃₀₇₄ → R; V₃₀₈₀ → I; S₃₁₈₇ → G; P₃₂₅₁ → R; V₃₂₆₀ → I; I₃₂₉₇ → V; V₃₃₄₂ → I; K₃₃₈₉ → E; Y₃₄₀₂ → D; N₃₄₀₆ → E	K₂₅₂₆ → R; M₂₆₄₁ → V; A₂₇₅₇ → G; G₂₇₅₈ → D; E₃₀₁₃ → G; Y₃₀₃₀ → H; V₃₀₈₀ → I; R₃₁₈₈ → G; P₃₂₅₁ → R; I₃₂₉₇ → V; V₃₃₄₂ → I; V₃₃₄₃ → A; R₃₃₈₄ → K; K₃₃₈₉ → E; Y₃₄₀₂ → D; N₃₄₀₆ → E	A₂₇₅₇; G₂₇₅₈; P₃₂₅₁; K₃₃₈₉; Y₃₄₀₂; N₃₄₀₆	No	16/16
Total			14	5	53/57

*Position number corresponds to the polyprotein sequence Glubinnoe/2004 of tickborne encephalitis virus (TBEV). CTHD, C-terminal hydrophobic domain. †The following polyprotein sequences were used for polyprotein alignment: DQ862460 (TBEV, Glubinnoe/2004); ABJ74160 (TBEV, 205); AAN73266 (TBEV, Senzhang); BAB71943 (TBEV, Oshima 5–10); BAB72162 (TBEV, Sofjin-HO); AF 069066 (TBEV, Vasilchenko);AAD34205 (TBEV, Vasilchenko); ABF46836 (TBEV, EK-238); AAO43537 (TBEV, Zausaev); NP_043135 (TBEV, Neudoerfl); AAZ80455 (TBEV, ts-variant of strain 263); AAA86739 (TBEV, 263); Q01299 (TBEV, Hypr); ABB90676 (Spanish sheep encephalitis virus); BB90675 (Turkish sheep encephalitis virus); AAQ91606 (OHFV, Bogolubovka); NP 878909 (OHFV, Bogoluvovska); AAR98531 (OHFV, Kubrin); ABB90677 (Greek goat encephalitis virus); NP_044677 (Louping ill virus);NP_620108 (Langat virus, TP21); AAF75260 (Langat virus, E5); P29837 (Langat virus, TP21);AAQ91607 (Kyasanur forest disease virus); NP_722551 (Alkhurma virus); NP_620099 (Powassan virus);AAL32169 (deer tick virus); ABB90669 (Gadgets Gully virus); ABB90671 (Karshi virus); YP_224133 (Karshi virus); ABE73208 (Karshi virus); ABB90673 (Royal Farm virus); ABB90670 (Kadam virus); ABB90674 (Saumarez Reef virus); ABB90668 (Meaban virus); ABB90672 (Tyuleniy virus); NP_689391). (Montana myotis leukoencephalitis virus). ‡For substitution in cleavage sites.