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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Nat Cell Biol. 2008 Apr 20;10(5):556–566. doi: 10.1038/ncb1718

Figure 7. DYN-1 is required for efficient recruitment of RAB-5 and RAB-7 to phagosomes containing engulfed apoptotic cells in the C. elegans gonad.

Figure 7

Bright field images represent DIC micrographs. Arrows and arrowheads indicate apoptotic germ cells or protein localized around apoptotic germ cells. dyn-1(ky51) worms are shown at the nonpermissive temperature (25 ÂC) unless otherwise stated. Scale bar, 10 Îm. n >10 for each transgenic. Error bars represent s.e.m.

Compared to wild type (aâd), CFP::RAB-5 (e,f) and YFP::RAB-7 (g,h) halos are decreased in the gonad of dyn-1(ky51) mutant worms (f,h, arrowheads); quantitated data is presented in (q). Further, phagosomes appear larger in dyn-1(ky51) mutant worms (e, g, arrowhead and volume quantitated in (s). Nematodes deficient in vps-34 (iâl) or rme-8 (mâp) showed similar decreases in the number of RAB-5 or RAB-7 corpses (quantitated in r).

(t) Schematic of the genetic pathway for phagosome maturation containing apoptotic cells, based on the data presented above and in earlier figures.