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. 2010 Apr 8;6(4):e1000898. doi: 10.1371/journal.pgen.1000898

Figure 1. Fmrp is expressed in aNPCs and new neurons in the adult DG, and the loss of Fmrp leads to increased aNPC proliferation.

Figure 1

(A) Fmrp is expressed in Sox2 (white) and Nestin (green) double-positive NPCs (arrowheads) in the granule neurons of the adult hippocampus. Arrowhead points to a positive cell located at the subgranular zone adjacent to the hilar region. (B,C) Fmrp is expressed in doublecortin (DCX)-positive (B, green) and NeuroD1-postive (C, green) newly generated neurons. Asterisks identify positive cells located at the subgranular zone adjacent to the hilar region. (A–C, Fmrp, red; Dapi, blue; Scale bars = 10 µm;). (D) aNPCs cultured under proliferating conditions expressed the neural progenitor markers Nestin (cytoplasmic, red) and Sox2 (nuclear, green; Dapi in blue). (E) Proliferating WT aNPCs, but not Fmr1 KO aNPCs, expressed Fmrp. (F) Both WT and KO aNPCs incorporate the thymidine analog, BrdU, under proliferating conditions (BrdU, red; Dapi, blue; (D,F), Scale bars = 50 µm). (G) Quantitative analysis showing that a higher percentage of Fmr1 KO aNPCs incorporated BrdU. (*, p<0.05; n = 3; Student's t-test; mean ± SEM).