Figure 5. Identification of the mRNAs regulated by Fmrp in aNPCs.

(A) Western blotting shows the amount of Fmrp in both input and immunoprecipitated Fmrp-containing mRNP complexes from both WT and Fmr1 KO aNPCs. (B) The RNAs from Input and from Fmrp-IP of WT and KO cells were isolated and subjected to cDNA synthesis and real-time PCR quantification. The results confirmed that Fmrp binds to the mRNAs of MAP1B, EF1a, CDK4, cyclin D1, and GSK3β in WT aNPCs. KO aNPCs and ß-Actin mRNA analyses were used as negative controls. (C) Representative western blotting image showing the protein expression levels of the target genes of Fmrp in both WT and Fmr1 KO aNPCs. EIF5 was used as a loading control for MAP1B, and ß-actin was used as a loading control for the others in western blots. Quantification of western blot band intensities is shown in Figure S6A.