Figure 2.

α-Synuclein exacerbates DA or DOPA toxicity in M17 cells. Overexpression of WT or A30P α-synuclein produces increased sensitivity to DA or DOPA. Cells were exposed to increasing concentration of catecholamines for 24 hr and cytotoxicity was subsequently estimated using the LDH assay (see Experimental Procedures). Cell lines included cells transfected with EV, WT or A30P α-synuclein. For each experiment, eight wells per concentration were used and each experiment was performed in triplicate. Values are the mean ± SEM. * p < 0.05