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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Biochemistry. 2005 Sep 6;44(35):11715–11721. doi: 10.1021/bi050942m

Figure 6.

Figure 6

Substrate kinetics of retinoid synthesis by chicken retina microsomes. (A) Retinoids synthesized following 2 min incubations at the indicated concentrations of atROL. Palm-CoA (100 μM) was added to each reaction mixture. (B) Retinoids synthesized following 2 min incubations at the indicated concentrations of 11cROL. Palm-CoA (100 μM) was added to each reaction mixture. (C) Retinoids synthesized following 2 min incubations at the indicated concentrations of palm-CoA. atROL (50 μM) was added to each reaction mixture. Data are expressed as initial reaction velocities (V0). The Vmax and KM constants derived from Eadie–Hofstee transformations of these data are presented in Table 1.