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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Biochemistry. 2005 Sep 6;44(35):11715–11721. doi: 10.1021/bi050942m

Figure 7.

Figure 7

Effect of apo-CRBP1 on retinoids synthesized from atROL by chicken retinal homogenates. (A) Chicken retina homogenates were preincubated with 55 μM CRBP1 before addition of 20 μM holo-CRBP1 (atROL substrate with CRBP1). Retinoids were extracted and analyzed by HPLC at the indicated incubation times. (B) Chicken retina homogenates were incubated under conditions similar to those used for panel A without apo-CRBP1 preincubation. Results are shown for each retinoid detected as nanomoles per milligram of protein. Error bars show standard deviations (n = 4). Note the synthesis of 11cROL from atROL with no net synthesis of atREs or 11cREs in panel A.