Table 1.
Vmax (nmol min−1 mg−1) | KM (μM) | |||||||
---|---|---|---|---|---|---|---|---|
substrate | 11cREs | 11cROL | atREs | atROL | 11cREs | 11cROL | atREs | atROL |
atROL | 0.82 | 0.19 | 0.28 | – | 13.5 | 10.1 | 10.9 | – |
11cROL | 0.17 | – | 0.04 | 0.14 | 12.1 | – | 13.0 | 35.0 |
palm-CoA | 0.99 | 0.23 | 0.27 | – | 34.4 | 28.5 | 13.2 | – |
Determinations for atROL and 11cROL were carried out at 100 μM palm-CoA. Determinations for palm-CoA were carried out at 50 μM atROL.