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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Biochemistry. 2005 Sep 6;44(35):11715–11721. doi: 10.1021/bi050942m

Table 1.

Kinetics of Retinoid Synthesis for atROL, 11cROL, and Palm-CoA Substratesa

Vmax (nmol min−1 mg−1) KM (μM)


substrate 11cREs 11cROL atREs atROL 11cREs 11cROL atREs atROL
atROL 0.82 0.19 0.28 13.5 10.1 10.9
11cROL 0.17 0.04 0.14 12.1 13.0 35.0
palm-CoA 0.99 0.23 0.27 34.4 28.5 13.2
a

Determinations for atROL and 11cROL were carried out at 100 μM palm-CoA. Determinations for palm-CoA were carried out at 50 μM atROL.