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. Author manuscript; available in PMC: 2011 Apr 2.
Published in final edited form as: Cell. 2010 Apr 2;141(1):94–106. doi: 10.1016/j.cell.2010.02.041

Figure 2. Prophase Phenotypes of msh4Δ Compared to WT.

Figure 2

(A-I) All chromosome axes are stained by Spo76-GFP.

(A-C) Mid-leptotene: in msh4Δ homologs are aligned at a greater distance (arrows in A and B) than in WT (arrow in C).

(D) Late leptotene of msh4Δ: one pair of homologs (arrowhead) is aligned at 200nm when others (arrow) remain at 600nm.

(E and F) Contrary to msh4Δ, WT homologs pair (C), start synapsis (E) and synapse (F) synchronously.

(G and H) Synapsis is completely asynchronous in msh4Δ. At zygotene (G) some homologs are half synapsed (arrowhead) while another pair is only synapsed at the telomere region the remainder of the axes remaining widely separated but aligned (arrows). At early pachytene (H) several or, as shown, one pair (arrow) remain(s) unsynapsed.

(I) At late pachytene Rad51-RFP foci (arrow) remain visible on non-synapsed regions of msh4Δ (arrow). Bars, 2μm.

See S1E and S1F for bouquet formation, S2C for chiasmata and Figures S3A-3G for Mer3 foci patterns in msh4Δ.