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. Author manuscript; available in PMC: 2011 Apr 2.
Published in final edited form as: Cell. 2010 Apr 2;141(1):94–106. doi: 10.1016/j.cell.2010.02.041

Figure 4. Mer3 and Mer3KA Localization.

Figure 4

(A - D) Colocalization of Mer3-GFP (A) and Rad51-RFP (C) foci at early leptotene with corresponding DAPI (B); (D) Merge of (A) and (C).

(E) Mer3 foci occur at matching sites (arrows) in the two aligned pairs of homologs seen in this optical section. (F) Corresponding pairs (arrows) stained by DAPI.

(G) Double Mer3 foci (arrow) among single foci; arrowhead points to the corresponding “empty” matching site; (H) Cartoon of foci (in red).

(I and J) Another example of Mer3 foci at matching sites with cartoon.

(K-M) Three examples of even-spaced Mer3 foci along leptotene chromosomes. At early leptotene (K and L) all foci are uniformly bright; from mid-leptotene on (M) some foci (arrows) are less bright than others.

(N) At early zygotene, co-staining with Spo76-GFP shows Mer3 foci (arrows) localized between axes in synapsed regions.

(O) Even-spaced Mer3KA foci at mid leptotene.

(P) mer3KA foci occur at matching sites (arrow) in aligned pair of homologs; (Q) corresponding DAPI. Bars, 2μm.

See Figures S3A-3G for patterns of Mer3 foci in msh4Δ.