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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Cell. 1985 Aug;42(1):39–50. doi: 10.1016/s0092-8674(85)80099-4

Figure 5.

Figure 5

Hydroxyapatite Chromatography of Squid Translocator Purified by Gel Filtration

Samples corresponding to fractions 28–32 from the gel filtration column shown in Figure 4 were pooled and applied to a 1 × 6 cm hydroxyapatite column equilibrated with gel filtration buffer at pH 7.4. Proteins were eluted with a 0–0.3 M phosphate gradient, and 0.5 ml fractions were collected. Microtubule-movement-inducing activity, assayed by serial dilution, correlated with the presence of 110 and 65 kd polypeptides in eluant fractions. Fraction 40 contains approximately 35 μg/ml of protein.