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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Cell. 1985 Aug;42(1):39–50. doi: 10.1016/s0092-8674(85)80099-4

Figure 6.

Figure 6

Cosedimentation of a 120 Kilodalton Bovine Brain Polypeptide with Squid Microtubules Depends upon AMP-PNP

Bovine brain S3 supernatant was incubated with squid optic lobe microtubules (100 μg/ml) in the absence (lane a) or presence (lane b) of 5 mM AMP-PNP for 15 min at 23°C. Microtubules were pelleted, washed once with PEM-taxol-GTP buffer, resuspended in 1/40 of the original volume with the same buffer containing 5 mM ATP and 0.1 M KCl, and then assayed for movement-inducing activity.