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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Cell. 1985 Aug;42(1):39–50. doi: 10.1016/s0092-8674(85)80099-4

Figure 7.

Figure 7

Gel Filtration Chromatography of the Microtubule-Purified Bovine Translocator

(A) Microtubule-purified bovine proteins (0.75 ml; purified as described in Experimental Procedures) were run on a Bio-Gel A5m column like that described in Figure 4, yielding the profile of ATPase and movement-inducing activities shown. (B) The polypeptide compositions of fractions 21–37 were analyzed by SDS polyacrylamide gel electrophoresis (40 μl per lane). (C) A densitometer scan of the Coomassie stained gel is shown of the lane corresponding to the fraction with greatest movement-inducing activity (30, which contains approximately 40–50 μg/ml of protein). Void (Vo) and excluded (VT) volumes are indicated.