Table 1.
Properties of Native Vesicle and Liposome Transport by Native DdUnc104 and Bacterially Expressed Minimotors
| Native Vesicles |
Liposomes (10 mol% PtdIns(4,5)P2) |
|||
|---|---|---|---|---|
| Movements (per minute per axoneme) | Velocities (μm/s) | Movements (per minute per axoneme) | Velocities (μm/s) | |
| DdUnc104 | 3.3 ± 0.6 | 2.1 ± 0.4 | 4.1 ± 1.0 | 2.0 ± 0.6 |
| CeU653-GFP | 0.1 ± 0.1 | 0.5 ± 0.4 | 0.1 ± 0.1 | 0.4 ± 0.3 |
| CeU653-PHDdUnc104 | 7.8 ± 1.5 | 1.5 ± 0.2 | 54.8 ± 6.5 | 1.6 ± 0.1 |
| CeU446-PHDdUnc104 | 5.6 ± 1.2 | 1.4 ± 0.2 | 45.4 ± 4.3 | 1.4 ± 0.2 |
| PHDdUnc104-Ncd | 1.5 ± 0.6 | 0.15 ± 0.04 | 1.2 ± 0.5 | 0.17 ± 0.06 |
The minimotors and the assay conditions are described in Figure 2 and the Experimental Procedures. The means and standard deviations from 3–5 independent experiments are shown.