Figure 5. CquiOR2 + CquiOR7 is highly responsive to indole.

A) Upper trace, an oocyte expressing CquiOR2 + CquiOR7 is challenged with 20 s applications of a range of concentrations of indole. Middle trace, an oocyte expressing CquiOR2 + CquiOR7 is challenged with 20 s applications of a range of concentrations of 3-methylindole. Lower trace, an oocyte expressing CquiOR2 + CquiOR7 is challenged with 20 s applications of a range of concentrations of 2-methylphenol. B) Concentration-response relationships for CquiOR2 + CquiOR7 expressing oocytes when activated with a range of indole, 3-methylindole and 2-methylphenol concentrations. All data are normalized to the response of each oocyte to 300 nM indole and the curves were fit as described in Materials and Methods (means ± SEM; n = 5–16). EC₅₀ and n_H values are provided in Table 1.