Figure 2. GF120918 treatment increases parasite motility.

A. Parasites were treated with 10 µM GF120918 (GF, white bars), 1 µM Ca²⁺ ionophore A23187 (CaI, gray bars) or the respective solvents (cntl) and the trails deposited during gliding enumerated. Data are average of trail number per field ± SE (n = 15) of a representative of 3 experiments and expressed as percentage of cntl. B. Indirect immunofluorescence microscopy (left panel) and trail length quantification (right panel) demonstrating that the average length of trails increased with GF120918 (GF) treatment. Data are percentage of short (<10 µM) or long (>10 µM) trails per field ± SE (n = 10) of a representative of 2 experiments. C. Parasites were treated with 10 µM GF120918 (GF) in presence or absence of 100 µM BAPTA and trail deposition was quantified as before. Data are average of trail number per field ± SE (n = 10) of a representative of 2 experiments and expressed as percentage of cntl-treated parasites. *, p<0.05.