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. 2010 Apr 8;5(4):e10062. doi: 10.1371/journal.pone.0010062

Figure 3. GF120918 treatment increases MIC2 secretion.

Figure 3

A. Isolated parasites were treated with 10 µM GF120918 (GF) or solvent (cntl) for 5 or 30 min, fixed and stained with anti-MIC2 antibody without cell permeabilization. Central panel, magnification of T. gondii treated with GF120918 for 30 min showing MIC2 surface staining. Insets, differential interference contrast images. B. Isolated parasites treated with 10 µM GF120918 (GF) or solvent (cntl) for 30 min were stained with anti-MIC2 antibody without (thick lines) or with (thin lines) cell permeabilization and quantified by flow cytometry. Overlay histogram showing cells treated with solvent (cntl, yellow lines) or GF120918 (GF, black lines). U, unstained cells.