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. 2010 Apr 8;6(4):e1000849. doi: 10.1371/journal.ppat.1000849

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A–D. A cytometric bead array was used to quantify the protein levels of cytokines in lung homogenates after infection with the Urbani virus. Lung samples were homogenized and protein levels of (A) TNFα, (B) IFNγ, (C) MCP1 and (D) IL6 were quantified. E–F. Real-time PCR analysis was performed on RNA from infected mouse lungs. Mice were harvested at days 2, 5 and 9 post-infection and lung RNA isolated by Trizol. cDNA was made from 1ug of RNA of each and used in real-time analysis and the results for 3 mice per group were combined. Fold increase over mock-infected lungs of each cytokine is graphed for (E) TNFα, (F) IFNγ, (G) MCP1 and (H) IL6.