Figure 4.

HIF-1α protein expression in THP-1 monocytes and macrophages. Protein expression of HIF-1α measured by Western Blotting in nuclear extracts of THP-1 cells. (A) HIF-1α expression during differentiation of THP-1 monocytes to macrophages in unstimulated and LPS-stimulated cells (6 hours). (B) Effect of different concentrations (2,10 or 50 μM) of kinase inhibitors on HIF-1α expression in LPS-stimulated THP-1 macrophages (cells were differentiated for 72 hours with 100 nM PMA): PD = PD98059-MEK inhibitor, LY = LY294002-PI3K inhibitor, KN = KN93-CAMKII inhibitor.