Table 1. Primers used for Real Time PCR for different tight junction genes.

Tight junction genes	Primer names	Primer sequence 5′ - 3′	References
ZO-1	ZO-1F	TGTGAGTCCTTCAGCTGTGGAA	Pu H, Tian J, Andras AS, Hayashi IK, et al, (2005) HIV-1 Tat protein-induced alterations of ZO-1 expression are mediated by redox-regulated ERK 1/2 activation. J Cerebral Blood Flow & Metabolism 25:1325–1335
	ZO-1R	GGAACTCAACACACCATTG
Occludin	Occlu F	CATTGCCATCTTTGCCTGTG	Bai L, Zhang Z, Zhang H, et al. (2008) HIV-1 Tat protein alter the tight junction integrity and function of retinal pigment epithelium: an in vitro study. BMC Infec. Dis..8:77–89
	Occlu R	AGCCATAACCATAGCCATAGC
Claudin 1	Claudin 1F	CAGGCTACGACCCGAAC	Bai L, et al (2008) BMC Infec. Dis..8:77–89
	Claudin 1R	CAGGCTACGCAAGGA
Claudin 2	Claudin 2F	CCCAAACCCACTAATCACATC	Bai L. et al (2008) BMC Infec. Dis..8:77–89
	Claudin 2R	GCCACTGCTTCTCCTTCC
Claudin 3	Claudin 3F	CAGGCTACGACCGCAAGGAC	Bai L. et al (2008) BMC Infec. Dis..8:77–89
	Claudin 3R	GGTGGTGGTGGTGGTGTTGG
Claudin 4	Claudin 4F	GGCGTGGTGTTCCTGTTG	Bai L. et al (2008) BMC Infec. Dis..8:77–89
	Claudin 4R	AGCGGATTGTAGAAGTCTTGG
Claudin 5	Claudin 5F	TACCGCAGGAAGAGGAGCAG	Bai L. et al (2008) BMC Infec. Dis..8:77–89
	Claudin 5R	GCCCGAAGCAGCCAATCC
GAPDH	GAPDH F	TCTCTGCTCCTCCTGTTC	Bai L. et al (2008) BMC Infec. Dis..8:77–89
	GAPDH R	CTCCGACCTTCACCTTCC