Fig. 1.

(A) Heat map based on maternal diet effects on placental gene expression. Placentae from dams on the C diet are well separated from the placentae of conceptuses from dams on the LF and VHF diets. Despite the differences in caloric density between two defined diets, the gene regulation patterns in placentae of LF and VHF conceptuses were more like each other than like those in placentae of C conceptuses. Gene tree clustering on 1,972 genes, whose expression was changed by >2-fold with P < 0.05, is able to distinguish placentae from dams on the LF and VHF diets. (B) Heat map based on fetal sex effects on placental gene expression. Placentae gene expression patterns of male conceptuses clearly clustered separately from the placentae of females, when data on the total regulated genes (with 2-fold differences) across all dietary groups are compared (P < 0.05). Samples marked with an asterisk are those from the left as opposed to the right uterine horn. The close grouping of these with the other samples suggests that uterine implantation site has no affect on placental gene expression patterns.