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Distribution of injected plasmid DNA and EGFP mRNA expression in each organ. (A) After 24 hr injection, plasmid DNA was detected at several organs in the TPFE and the Lipofectin group (n = 6 for each group). (B) mRNA expression of EGFP gene was analyzed using real-time-PCR at 24 h after injection. mRNA was detected at the lung, liver, and spleen (n = 6 for each group). *p < 0.05 versus the Lipofectin group. Error bars are SEM.
