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Characterization of RAS1 expression and T-DNA insertion mutant. (A) RAS1 expression in Sha, NIL, and Ler. (B) RAS1 expression in the T-DNA line and its WT. Seedlings were treated with 150 mM NaCl or 100 μM ABA for 0–2 h. TUB4 expression serves as the RNA loading control. (C) Germination and seedling growth of the T-DNA insertion mutant of RAS1 on MS medium supplemented with 150 mM NaCl or 2.5 μM ABA.
