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PEX1 and PEX6 protein levels in PEX1-214 cells homozygous for G843D that do not recover with chemical treatment. Lanes C, control; lane 1, untreated; lane 2, 200 mM TMAO; lane 3, 3% DMSO; lane 4, 10 μM AD; lane 5, 10 μM Epicholestanol; lane 6, GF109203x 3 μM; lane 7, Ro31-8220 0.5 μM; lane 8, 5% glycerol, 37 °C and 30 °C. Note that PEX1 and PEX6 levels increase slightly at 30 °C. GAPDH was used to evaluate protein loading.
