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. 2010 Mar 8;107(12):5605–5610. doi: 10.1073/pnas.0910578107

Fig. 5.

Fig. 5.

(A) DNMT1 mRNA levels were not affected by IH E2 infusion (5 μg/side). (B and C) DNMT3A and DNMT3B mRNA was significantly increased relative to Veh 45 min after IH E2 (**P < 0.01). (D) c-fos mRNA was significantly increased relative to Veh 15 min after IH E2 (**P < 0.01). (E) DNMT1 protein was not changed by IH E2 infusion. (F) DNMT3A protein was slightly, but not significantly, increased 3 h after IH E2. (G) DNMT3B protein was significantly increased relative to Veh 4 h after IH E2 (*P < 0.05). (H) Mice receiving ICV E2 (10 μg), but not Veh (ICV HBC + IH 0.8% acetic acid), immediately after training spent significantly more time with the novel object than chance (*P < 0.05) after 48 h; this effect was blocked by IH 5-AZA (100 μg/side). Mice receiving IH 5-AZA plus ICV Veh (n = 8) immediately after training spent significantly more time with the novel object than chance (*P < 0.05) after 48 h; this effect was time limited, as infusion of IH 5-AZA plus ICV Veh 3 h after training (n = 6) did not increase time with the novel object after 48 h.