Fig. 1.
Sequence of a FH and MH experiment in a drop of MpAFP solution. (A) A single ice crystal grown in 36 μM MpAFP solution was stable down to -1.87 °C below the melting point. (B) Dendritic growth indicating the sudden growth of the ice at this supercooling. The FH of the sample was determined as the difference between the temperature at which this dendritic growth commenced and the Tm of the sample. (C) Growth continued until most of the sample was frozen. (D–E) When the frozen sample was warmed to close to the melting temperature, all of the ice melted except for the initial crystal. (F) The remaining crystal was slowly warmed further. (G) The ice crystal remained stable at superheatings of up to 0.18 °C above Tm for over 25 min. (H) The crystal remained stable for 5 s at +0.18 °C before rapidly melting (I) in a 0.14 s time interval, with a velocity of 48 μm/s. The difference between the Tm and the temperature at which the crystal melted was taken as the MH.