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Fluorescence images of ice superheated in a GFP-MpAFP solution. (A–E) A series of images recorded while the crystal was warmed slowly to temperatures above the equilibrium melting point. (F) At a superheating of 0.04 °C, melting of the superheated ice crystal started at a point on the surface and proceeded through the crystal. (G–J) After melting commenced, the GFP-tagged AFPs that had been adsorbed on the ice diffused away.
