Table 3.

GPC, SLS, and ITC.

Molecular weights* calculated from GPC and SLS measurements
	pH	MWrh (kDa)	MWabs (kDa)
E1-HP	8.0	29	22.9 ± 0.2
E1+HP	8.0	60	42 ± 2
Binding parameters measured by ITC
	pH	Kd, μM	ΔH, kJ/mol	ΔS, J/K·mol	n†
E1+dp12	5.7	3.0 ± 0.1	+54 ± 4	+280±16	1.0 ± 0.1
E1+dp12	7.2	5 ± 1	+62 ± 6	+301±40	0.41 ± 0.06

^*MW_th = 21.7 kDa

^†The stoichiometric factor n corresponds to the molar ratio of heparin: APP–E1 in the complex.