Fig. 5.

CD4 T cells from Apc^Min/+ mice are functionally altered. (A) CD4 T cells from Apc^Min/+ mice were isolated and activated with given amount of anti-CD3 and T cell–depleted irradiated splenocytes for 72 h. (B) Foxp3+ regulatory T cells were isolated from Apc^Min/+ Foxp3-IRES-RFP mice and cocultured with Foxp3- CD4 effector T cells from either Apc^Min/+ mice or their littermate control for 72 h for given ratios. (C) CD4 T cells from Apc^Min/+ mice were isolated and activated for 96 h, and cytokines were measured by cytokine bead array. Data indicate means ± SD of three separate experiments as triplicates.