Fig. 4.

JNK exhibits all-or-none response to anisomycin. (A) Normalized emission ratio time courses from representative experiments of HeLa cells expressing JNKAR1 that were treated with different doses of anisomycin (5 μM, 500 nM, 50 nM, and 20 nM). (B) Normalized emission-ratio time courses of two JNKAR1 expressing cells that were treated with 100 nM anisomycin. (C) Scatter plot of JNKAR1 responses, shown in percentage-change of emission ratio of yellow-to-cyan, in individual HeLa cells, in response to various doses of anisomycin. (D) Imstain analysis of nuclear phospho-ATF-2 level, as detected by an anti-phospho-ATF-2 antibody, in HeLa cells stimulated with an intermediate concentration (70 nM) of anisomycin for different durations.