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Proviral integration and gene expression analysis by PCR. Stem cells from exfoliated deciduous teeth (SHED), stem cells from apical papilla (SCAP)-induced pluripotent stem (iPS) cells, and dental pulp stem cells (DPSCs)-iPS cells were harvestedfor DNA isolation and subjected to PCR for the detection of Lin28, Nanog, Oct4, and Sox2 transgenes in the genome. Passage numbers at cell harvesting: SHED-iPS cells, p10; SCAP-iPS cells, p8; DPSC-iPS cells, p12.
