Fig. 2.

CD34^highCD45RA⁻Lin⁻ pluripotent precursors develop into a CD34^highCD45RA^low/intLin⁻ intermediate stage that acquires either CD10 or CD19 in a mutually exclusive manner. (A) Magnetically enriched CB CD34^high cells were labeled with antibodies against CD34, CD45RA, CD10, and CD19 antigens, and CD34^highCD45RA⁻CD10⁻CD19⁻ or CD34^highCD45RA^low/intCD10⁻CD19⁻ populations gated as indicated (Left) were sorted. (Right) Reanalysis and purity of sorted cells (99.9 ± 0.1% and 99.0 ± 0.2%, respectively; n = 4). (B) CD34, CD45RA, CD10, and CD19 expression patterns in CD34^highCD45RA⁻Lin⁻ (Upper) and CD34^highCD45RA^low/intLin⁻ (Lower) progenies, respectively, after 2 weeks of S17 cocultures. The input cell number was 10³ cells per well, and the 2-week yield was 50.78 ± 6.9 × 10³ or 304.38 ± 45 × 10³ cells per well for CD34^highCD45RA⁻CD10⁻CD19⁻ or CD34^highCD45RA^low/intCD10⁻CD19⁻ progeny, respectively. Data are representative of four independent experiments.