Skip to main content
. 2010 Mar 10;107(13):5907–5912. doi: 10.1073/pnas.1001647107

Fig. 2.

Fig. 2.

TSPAN33 and TSPAN5 redundantly down-regulate NOTCH1 activation. (A–E) NOTCH/RBPJ luciferase reporter assays in HeLa cells expressing NOTCH1 L1601P-ΔPEST (NOTCH1 HD-ΔP) (A), NOTCH1 Jurkat JME17 (NOTCH1 JME17) (B), NOTCH1 P12 ICHIKAWA (NOTCH1 P12) (C), ΔEΔPEST-NOTCH1 (D), or the intracellular form of NOTCH1 (NOTCH1-ICN) (E) in the presence of a siRNAs targeting TSPAN33, TSPAN5, or a control inactive siRNA (control). NOTCH/RBPJ reporter activity after normalization with respect to the Renilla luciferase transfection control is as shown as relative levels compared to those present in control siRNA transfected cells. Quantitative RT-PCR analysis of TSPAN5 (orange bars) and TSPAN33 (blue bars) demonstrating reduced levels of expression upon siRNA knockdown compared with siRNA control and similar knockdown levels in single SiRNA and double siRNA transfected cells is shown. The changes in NOTCH/RBPJ reporter activity in HeLa cells expressing the different NOTCH1 alleles in the presence of TSPAN5 knock-down did not reach statistical significance except for the P12 ICHIKAWA mutant.