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. 2010 Mar 15;107(13):5943–5948. doi: 10.1073/pnas.0911755107

Fig. 1.

Fig. 1.

Enforced expression of Notch in T cells induces IL-22. (A) Total spleen cells from C57BL/6 mice were stimulated with soluble anti-CD3 mAb (1 μg/mL) for 24 h and transduced with a retrovirus carrying N1ICD, N2ICD, N3ICD, or a control virus. Cells were further stimulated with anti-CD3 mAb (1 μg/mL) for 48 h. Then, the expression of Il22 in MACS-enriched CD4+ T cells was analyzed by real-time PCR. (B) Total spleen cells were stimulated with anti-CD3 mAb in Th0, Th1, Th2, and Th17 conditions for 24 h and then transduced with N2ICD or control virus. After 48 h of further culture under the same conditions, Il22 and Il17a expression in MACS-enriched CD4+ T cells was measured by real-time PCR. (C) ELISA for the detection of IL-22 secretion from naive CD4+ T cells (CD4+CD62L+) isolated from OT-II TCR transgenic mice stimulated with OVA peptide-pulsed Cont-DC (open) or DL1-DC (filled) for 3 days. (D) ELISA for the detection of IL-22 secretion from naive CD4+ T cells isolated from STAT3F/F or STAT3F/F-Cre transgenic mice stimulated with allogenic Cont-DC (open) or DL1-DC (filled) prepared from BALB/c mice. *, P < 0.05, indicates a statistically significant difference. Data are representative of at least four independent experiments. N.D., not detected; N.S., not significant.