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Spectral tuning in PYP. (A) Structure of the pCA chromophore in the native state of PYP with active site residues Tyr42 and Glu46. (B) Amplitude-normalized absorbance spectra for free pCA, pCA attached to fully unfolded PYP in its neutral (pU) and ionized (pU-) state, and PYP in its native pG state. All four samples are in aqueous solution. Note that besides the absorbance maximum also the shape and width of the absorbance band is affected.
