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Correlation of excited state lifetime and fluorescence quantum yield Φfl in wt PYP and the E46H, E46K, and E46Y mutants. (A) Determination of excited state lifetime by sub-ps transient absorbance pump-probe spectroscopy of wt PYP (black line) and its E46H (blue line), E46K (green line), and E46Y (red line) mutants. (B) Correlation between excited state lifetime and Φfl in wt PYP and the three mutants. The error bars depict the standard deviations.
