Table 3.
Receptor Ligand | koff, min−1 | kon, 109 M−1 min−1 | Kd, nM |
A−AChBP | |||
SPX | 0.041 ± 0.023 | 2.2 ± 0.7 | 0.019 |
GYM | 0.038 ± 0.013 | 8.1 ± 1.3 | 0.0047 |
L−AChBP | |||
SPX | 0.82 ± 0.25 | 0.69 ± 0.07 | 1.2* |
GYM | 0.0037 ± 0.0013 | 2.8 ± 0.9 | 0.0013 |
Values are means ± SD from 3–6 measurements.
*A Kd of 1.26 ± 0.11 nM was alternatively determined by equilibrium titration using scintillation proximity assay for this lower affinity complex.