Table 1.

Sensitivity of human, murine, and canine CENP-E motor domains to GSK923295 and reduction in sensitivity of human CENP-E after mutation of I182 and T183 to corresponding murine residues

Species	Mutation	GSK923295 Ki (nM)
Human	wt	3.2 ± 0.2
Canine	wt	1.6 ± 0.1
Murine	wt	67 ± 5
Human	I182L T183A	61 ± 8
Human	I182L	14 ± 0.2
Human	T183A	13 ± 0.2

K_i values are ± SD (n = 4). Sequence comparison of human, canine, and murine CENP-E motor domains is shown in Fig. S4 and characterization of mutant enzymes in Table S3.