Fig. 4.

Ectopic expression of transitional neocortex-specific genes in the Zbtb20^−/− CA1 region. Coronal forebrain sections were detected for indicated genes by immunohistochemistry (A and B) or in situ hybridization (C–L) at the indicated stages. The arrowheads in B and D indicate the mutant CA1 field. The star in C indicates the notch at the DG crest. The arrowheads in H and J indicate the deep and upper layer of the mutant CA1, respectively. The dotted lines in H and J outline the upper and deep boundary of mutant CA1, respectively. (Insets) Low-magnification views of the hippocampus (A–D and I–J). (K and L) At caudal level, the expression domain of Fibronectin1, a marker for subiculum (K), was shifted adjacent to the mutant CA3 (L). (Scale bars: 200 μm for A–D; 250 μm for E–L.)