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Selection of high affinity Mac-1 I domains. (A) A mutagenesis library of Mac-1 I domain in yeast was screened with HeLa cells to isolate high affinity mutants. Yeast cells appear as small bright spheres in the images. (B and C) SPR measurement of the binding of active I domains (I316G and F302L) to ICAM-1, iC3b, and fibronection. I domains were injected at a series of two-fold serial dilutions starting at 250 nM.
