Fig. 3.

Selection of phage library against the F302L displayed in yeast. (A) A phage library after each round of panning was examined for binding to the F302L and the wild-type I domain using flow cytometry. Unless noted otherwise, open and filled histograms correspond to antibody binding to uninduced and induced cells, respectively. Control indicates yeast cells expressing unrelated proteins. (B) The cDNAs amplified from the second and fourth round sort were introduced into the YS2H vector along with the F302L for final selection of antibodies. (C) Selected individual clones exhibit differential level of Myc expression, correlating with antibody-F302L binding affinity.