Fig. 6.

Evaluation of antibodies in inhibition of neutrophil adhesion and migration. (A) Activation-dependent binding of antibodies to neutrophils was examined with immunofluorescence flow cytometry. The histograms represent antiHis antibody binding with (Filled) and without (Open) antibodies against Mac-1. (B) The inhibition of neutrophil adhesion to fibrinogen by antibodies was measured using 96-well V-bottom plate assays. Student’s t tests were used to determine statistical significance (p < 0.05 for * vs. control). (C) The migration of neutrophils on fibrinogen-coated surface after10 nM fMLP activation was recorded and analyzed. Each line represents the movement of single neutrophils.