Fig. 3.
Immunoblot analysis to evaluate the amounts of peroxiredoxin and thioredoxin proteins expressed in T. pallidum. Estimated expression levels of AhpC, Trx, and FlaA in T. pallidum harvested from rabbit testes (left side of gel) based on purified recombinant protein standards (right side) are given as the total amount of native protein (ng) in 2.5 × 107 treponemes (averages ± standard deviations calculated from four samples out of a single harvest). The lower mobility of recombinant AhpC and FlaA reflects the additional His tag on these proteins. Intracellular protein concentrations were calculated based on the estimated volume of T. pallidum (2.54 × 10-16 L) (SI Experimental Procedures). Concentrations shown for TpAhpC and TpTrx correspond to 6.3 and 1.2 mg/mL in the cell, respectively. Numbers to the left of the immunoblots represent molecular weight standards in kilodaltons.