Fig. 4.

MIPs are expressed in the CNS but not in reproductive organs. Antibody against MIP detected MIP-positive neurons and their arborizations in the brain (A) and the ventral nerve cord (B). In contrast, no MIP immunoreactivity was detected in male reproductive organs of Drosophila (C’) or Aedes (D’). GFP (C’’) and DAPI (D’’) were used for counterstaining in Drosophila (w;UAS-lamin-EGFP;Fru-GAL4) and Aedes respectively. Ag, accessory gland, de, ejaculatory duct, te, testis. (Scale bar, 100 μm.)