Skip to main content
. 2010 Mar 22;107(14):6204–6209. doi: 10.1073/pnas.0914423107

Fig. 1.

Fig. 1.

Secondary structural models for synthetic riboswitches tested in this study. Nucleotides changed with respect to the original sequence are shown in red. SD sequences are boxed. Restriction sites (BamHI, NsiI) are underlined. The start codons are part of NsiI restriction sites. All switches are designed as translational “on” switches and are shown in the proposed off mode (i.e., in the absence of the metabolite). In this mode the SD sequence is masked and, therefore, inaccessible to the ribosome. (A) A synthetic glycine riboswitch (s.gly-RS) containing an anti-Shine-Dalgarno (ASD) sequence designed to allow translational regulation by base-pairing with the SD sequence. The switch is derived from the glycine riboswitch (gly-RS) from Bacillus subtilis (8) (Fig. S1A). (B) A synthetic adenine riboswitch (s.ade-RS) designed as translational “on” switch. The switch is derived from the ydhL adenine riboswitch (ade-RS) from Bacillus subtilis (9) (Fig. S1B). (C) The synthetic theophylline responsive riboswitch (s.theo-RS) based on helix slipping (12). The one-nucleotide theophylline-induced slipping in the secondary structure is indicated by the red arrow. The 5′UTR contains two possible SD sequences (boxed).