Fig. 1.

Xbsx expression during pineal organ development. Xbsx whole-mount in situ hybridization was performed at stage 16 (A), stage 19 (B), stage 22 (C), stage 32 (D), and stage 38 (E). Immunostaining for BrdU incorporation (G, J, M, and P; green staining), in situ hybridization for Xbsx (F, I, L, and O; red staining), and merge (H, K, N, and Q) were performed on cryostat sections at stage 24 (F–H), stage 32 (I–K), stage 37 (L–N), and stage 40 (O–Q). In situ hybridization for Xbsx (R, red staining), Xotx5 (S, blue staining), and merge (T) was performed on stage 37 cryostat sections. In situ hybridization for Xbsx (U, red staining), Hermes (V, blue staining), and merge (W) was performed on stage 37 cryostat sections.