Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2010 Apr 9.

Published in final edited form as: J Muscle Res Cell Motil. 2009 Feb 10;30(1-2):41–55. doi: 10.1007/s10974-009-9173-1

Expression of LPP and palladin is up regulated by heat-denatured collagen compared to native collagen. a): R518 cells were cultured on coverslips coated with native and heat denatured collagen or directly on glass for 24 h. Following fixation, LPP, palladin, phosphoFAK, and TN-C were detected by immunofluorescence as detailed in the Methods section. Actin was stained with Alexa fluor 488-conjugated phalloidin. (Column a, c, e ) Scare bar, 20 µm. Cells shown are representative of the population. b): western blot comparing the expression of LPP, palladin, TN-C and pFAK. R518 cells were cultured on native and heat denatured collagen gels. Equal number of cells were loaded for gel. Data is representative of three experiments

Expression of LPP and palladin is up regulated by heat-denatured collagen compared to native collagen. a): R518 cells were cultured on coverslips coated with native and heat denatured collagen or directly on glass for 24 h. Following fixation, LPP, palladin, phosphoFAK, and TN-C were detected by immunofluorescence as detailed in the Methods section. Actin was stained with Alexa fluor 488-conjugated phalloidin. (Column a, c, e ) Scare bar, 20 µm. Cells shown are representative of the population. b): western blot comparing the expression of LPP, palladin, TN-C and pFAK. R518 cells were cultured on native and heat denatured collagen gels. Equal number of cells were loaded for gel. Data is representative of three experiments