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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: FEMS Yeast Res. 2009 Dec 18;10(2):168–176. doi: 10.1111/j.1567-1364.2009.00601.x

Figure 2.

Figure 2

Figure 2

UTH1 is involved in maintaining the integrity of the yeast cell wall. (A) Deleting UTH1 enhances cell growth on media supplemented with calcofluor white (CFW) or sodium dodecyl sulfate (SDS). 5 μl aliquots of 10-fold serial dilutions of wildtype and Δuth1 mutant cells in the W303-1A strain background were plated onto the designated media and cultured at 30°C for 2 or 4 days, depending upon the particular plate. YPDS denotes YPD plates supplemented with 1 M sorbitol respectively. (B) Overexpression of PKC1 enhances cell wall integrity mimicking a deletion of UTH1. 5 μl aliquots of 10-fold serial dilutions of wildtype and Δuth1 mutant cells transformed with plasmid pFR22 to overexpress PKC1 were plated onto the designated selective SD plates and cultured at 30°C for 2 or 5 days. SD-S denotes SD plates supplemented with 1 M sorbitol. All spot assays were repeated at least three times and a representative experiment is shown.