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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: FEMS Yeast Res. 2009 Dec 18;10(2):168–176. doi: 10.1111/j.1567-1364.2009.00601.x

Figure 3.

Figure 3

Deleting UTH1 decreases the rate of spheroplast formation of yeast cells. Cells of the indicated genotypes were cultured overnight in rich YPD media at 30°C, harvested and washed three times with deionized water, and then resuspended to an OD660 of 0.5 in TE buffer, pH 7.5 (50 mM Tris/HCl, 5 mM EDTA). Zymolyase (5 U/μl; Zymo Research, Orange, CA) was then added to the cells to a final concentration of 12 μg/ml. Cell suspensions were incubated at 23°C and their optical density (OD660) was recorded at four-minute intervals for the indicated time period. Assays were done in triplicate and mean values are shown along with the standard deviation.