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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Int J Oncol. 2010 May;36(5):1165–1173. doi: 10.3892/ijo_00000599

Figure 1.

Figure 1

Human umbilical cord blood cells were isolated by standard Ficol gradient separation. To separate stem cells (hUCB) from the nucleated cell population of the umbilical cord blood, cells were labeled with anti CD44-FITC and anti CD44-Tx Red antibody and separated by flowcytometry (A). To determine the ability of the isolated cells to form embryoid bodies, cells were cultured in media containing methyl cellulose (B). To determine the interaction of hUCB with glioma cells, a modified Boyden’s chamber was used. The chamber consisted of a porous membrane (0.22 μm pore size), which was coated with Matrigel to facilitate the growth of cells on both the upper and lower surfaces. Human umbilical cord blood stem cells were grown on the upper surface and glioma cells (SNB19, U87, 5310 or 4910) were grown on the lower surface (C).